Journal: Journal of Inflammation Research
Article Title: Association of Increased Nasal Fluids-Serum Concordance of Protein Profile with Prognosis in Nasal Polyps
doi: 10.2147/JIR.S567454
Figure Lengend Snippet: Expression validation of LIFR in CRSwNP. ( A ) Transcriptional levels of biomarkers (LIFR, LIF, IL-5, IL-13) in PR versus non-PR groups. ELISA quantification of LIFR concentrations in both ( B ) serum and ( C ) NF of PR patients compared to non-PR and control groups. ( D ) Representative images of H&E and LIFR immunohistochemical staining (Magnification ×400). ( E ) Quantification of LIFR protein expression intensity (n=6/group). ( F and G ) scRNA-seq analysis of CRSwNP tissues showing LIFR expression patterns across cell populations (left: cell type clusters; right: LIFR expression). ( H ) Immunofluorescence of LIFR (red) co-localization with vascular endothelial cells (CD31+, green) and nuclear staining (DAPI, blue) at ×400 magnifications (n=6/group). * P < 0.05, ** P < 0.01, **** P < 0.0001.
Article Snippet: For immunofluorescence, endothelial cells in nasal mucosa were marked using an anti-CD31 antibody (Bioss #bsm-10825M).
Techniques: Expressing, Biomarker Discovery, Enzyme-linked Immunosorbent Assay, Control, Immunohistochemical staining, Staining, Immunofluorescence